Caracterização química e estudo biológico da biomassa do fungo I1SYS1A1 isolado a partir de amostras de solo mineralizado

Abstract

The fungi comprise a heterogeneous group of heterotrophic microorganisms that act as saprobes, parasites or, less frequently, as symbionts living in association with other organisms. Considering the lack of studies in Brazil dealing with filamentous mycota as well as the need for knowledge of the diversity of these fungi, this study aimed to chemical and biological study of I1SYS1A1 lineage that was isolated from mineralized soil sample collected in Canaã dos Carajás/PA. To obtain the fungal biomass cultivation was performed on solid environment (rice). The ethanol extracts obtained from biomass were submitted to the characterization tests (screening) and classical chromatography looking for new substances that may present useful biological properties. In this sense, were performed cytotoxic and allelopathic bioassays. The evaluation of allelopathic potential was performed using seeds of the species Senna obtusifolia (obtusifolia) and Mimosa pudica (malice), aimed at inhibiting seed germination; in cytotoxicity assays, tumor cell lines HCT-116 (human colon), AGP (gastric ascites), MCF7 (breast, human), SKMEL (human melanoma), and normal line MRC5 (human fibroblast) were used. Tests for chemical characterization led to finding of steroids. The extract showed satisfactory results in the allelopathic test with malice seeds. There was no activity of the extract with the tested tumor cells. In the chromatographic separation were obtained two substances, one being ergosterol, which is in agreement with the chemical characterization undertaken. Currently work is underway in an attempt to identify other substances.