Avaliação do potencial citotóxico e identificação de citocalasinas isoladas de I8FDN, um endofítico de Derris nicou

Abstract

Cancer is characterized by the rapid and uncontrolled growth of cells that can invade tissues and surrounding organs. Currently, cancer is a worldwide public health problem. The research of natural products as compounds having biological activities has provided drugs in clinical use with great therapeutic potential, including chemotherapy for cancer. Endophytic fungi are excellent producers of bioactive metabolites. Based on this, the objective of this study was to identify the isolated Cytochalasins of the I8FDN fungus associated as endophytic of Derris nicou leaves as well as to evaluate the potential cytotoxic and hemolytic in vitro of two Cytochalasins front of tumor cell lines HCT-116 (human colon), AGP01 (gastric ascites), MCF7 (breast-human), SKMEL19 (human melanoma), and a non-tumor, MRC5 (human fibroblast) using the MTT assay (3- (4,5-dimethyl-thiazol-2) - 2,5-diphenyl-2 Htetrazolium bromide) and hemolysis assay in mice. The data show that the C1 compound showed high cytotoxicity in all lineages tested after 72 hours of treatment being more selective for the lineage SKMEL 19 with CI50/ml of 0,4137 μg. Moreover, cytochalasin D significantly inhibited cell growth in AGP01, MCF7 and SKMEL19 lines after 72 hours of treatment likewise showed more selective activity for the line SKMEL 19 with CI50 0,1685μg/ml. The substances did not cause lysis in the erythrocytes membrane. The results suggest that the tested Cytochalasins may be a potential agent for the treatment of melanoma, however, further studies should be conducted to evaluate the efficacy and selectivity for tumor cells.